Part:BBa_K1965050
P5:GS6:PPVs:GS6:cLuc:HA
This construct consists of a C-terminal part of the split luciferase that is fused to the P5 coiled coil via a linker which contains the cleavage substrate for PPV protease. The construct also contains the HA tag on the C-terminus. The P5 coiled coil dimerizes with the AP6 and P6 coiled coils. If its partner coiled coil (AP6 or P6) contains the N-terminal part of the split luciferase, then dimerization of the two coils enables luciferase reconstitution due to the close proximity of the split luciferase parts. However, because the linker contains PPV protease cleavage substrate, the cLuc can be cleaved off in the presence of the PPV protease, preventing reconstitution of the split luciferase.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 139
- 1000COMPATIBLE WITH RFC[1000]
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